Reactions examined with P450s 2S1 and 2W1 Each reaction was catalyzed, with the indicated substrate concentration, using E. coli membranes in which the P450 was coexpressed with human NADPH-P450 reductase. The limits of detection are indicated.
| Reaction | P450 2S1 | P450 2W1 |
|---|---|---|
| nmol product formed/min/nmol P450 | ||
| d-Benzphetamine N-demethylation (1.0 mM) | <0.02 | 2.9 |
| N,N-Dimethylnitrosamine N-demethylation (1.0 mM) | <0.02 | <0.02 |
| Naphthalene oxidation (300 μM), (± excess epoxide hydrolase; to 1- and 2-naphthol, 1,2-dihydronaphthol, or any other products) | <0.05 | <0.05 |
| Testosterone hydroxylation (100 μM) | <0.08 | <0.08 |
| Benzo[a]pyrene oxidation (100 μM) | <0.3 | <0.05 |
| All-trans-retinoic acid oxidation (10 μM) | <0.003 | <0.003 |
| Oleic acid hydroxylation (9 μM) | <0.003 | <0.03 |
| Arachidonic acid hydroxylation (50 μM) | <0.01 | ∼0.05 |