Selection of N-terminal sequences of P450s 2S1 and 2W1 for heterologous expression Amino acid changes are in italics and underlined.
| Construct | Basis of Selection of N Terminus | N-Terminal Amino Acid Sequence |
|---|---|---|
| 2S1 Native | MEATGTWALLLALALLLLLTLALSGTRARGHL PPGPTPLP | |
| 2S1 #1 | P450 2C3 rabbita | MAKKTSSKGKL PPGPTPLP |
| 2S1 #2 | P450 2C11 ratb | MARQSFGRGKL PPGPTPLP |
| 2S1 #3 | P450 2E1 humanc | MARQVHSSWNL PPGPTPLP |
| 2S1 #4 | P450 17A1 bovined | MALLLAVFLLLLLTLALSGTRARGHL PPGPTPLP |
| 2S1 #5 | Full-length | MAATGTWALLLALALLLLLTLALSGTRARGHL PPGPTPLP |
| 2W1 #1 | Synthesized native | MALLLLLFLGLLGLWGLLCACAQDPSPAARW PPGPRPLP |
| 2W1 #2 | P450 17A1 bovined | MALLLAVFLGLLGLWGLLCACAQDPSPAARW PPGPRPLP |
| 2W1 #3 | P450 2C3 rabbita | MAKKTSSKGKL PPGPRPLP |