Primers used for amplification of CYP2C-positive BAC clones
| Gene | Sequence (5′→3′) | |
|---|---|---|
| Forward | Reverse | |
| CYP2C20 | ||
| 5′ | ATGGAACCTTTTGTGGTCCTG | GAAAGATTTGCAGATGTCCTTAAC |
| 3′ | TTTGTGCAGGAGAGGGACTT | ACGAGGGTGGCAGAGAAAT |
| CYP2C43 | ||
| 5′ | TCTTGAAGCTGGGTATTGGTC | AGGTGGATCACAAGGTCAGG |
| 3′ | GGATAAAATTATCCTCAAAATCCTC | CATCAAAGGTCACAGAATAAAGG |
| CYP2C75 | ||
| 5′ | TTGTTGCCTTTTCTCCATCA | CACGGTTAAACCATCTTTCACA |
| 3′ | GTTAAAGGAGATAATGAGCCACAG | GGAAAGATGTGTTTGCTTCCAC |
| CYP2C18-like | ||
| 5′ | GTGAAAGCCCACAGTTTTCTTAC | CTCATGTCCTTAACATCTAACTGC |
| 3′ | TGACATCACCCCCATTGC | TGTGGGTTGACAAGTCAGAG |
| CYP2C76 | ||
| 5′ | CCCAGCAATGGATCTCTTCA | TGGCACCAAGCATTTATCTC |
| 3′ | CTTGCCTGTGTGTCAACCAT | ATTGGAATGGATTTTGAAGGA |