Primers and probes used for real-time RT-PCR
| Gene and Primer/Probe | Sequence (5′ → 3′) |
|---|---|
| CYP2C20 | |
| Forward | TTTCTGGAAGAGGCATTTTGC |
| Reverse | TCCATCTCTTTCCATTGCTGG |
| Probe | AACGGACTTGGAATCA |
| CYP2C43 | |
| Forward | GCCATTTCCCACTGTTTGAAA |
| Reverse | GCAGCGTCATGAGGGAGAA |
| Probe | ACAATTCCAAATCTTCT |
| CYP2C75 | |
| Forward | TTCCATTGGCTGACAGAGCTAA |
| Reverse | CCGCAGTGTCATGAGGGAA |
| Probe | CGATTCCAAATCCT |
| CYP2C76 | |
| Forward | TGGCCGAGGGAGTTTTCC |
| Reverse | AGAGAGAAACGCCGAATTTGC |
| Probe | CCAAGGATTCGGAGTTA |