TABLE 4

Time course of TCDD-induced gene expression changes associated with heart development and function in zebrafish larvae

Bold values represent real-time PCR results.

Affymetrix ID Gene Title Log2 (TCDD/DMSO)
1h 2h 4h 12h
Ion regulation
Dr0.13774.1.S1_ata Similar to sphingosine 1-phosphate receptor Edg-3b 1.9 2.7 1.9 1.8
Dr0.9896.1.A1_at Similar to serum/glucocorticoid regulated kinaseb 1.5 2.6 2.1 1.7
Dr0.10713.1.S1_at Na+/K+ ATPase α1a3 polypeptide 1.7 NC NC NC
Dr0.9878.1.S1_at Na+/K+ ATPase α3b polypeptide NC NC 1.5 1.7
NC 1.5 3.5 3.0
Dr0.14133.1.S1_at Similar to K+ channel tetramerization domain NC 1.3 NC 1.1
Dr0.3021.2.S1_at Similar to regulator protein p122-RhoGAP NC 1.1 NC NC
Dr0.1435.1.S1_at Caveolin 3 NC NC 1.2 1.9
Contractility
Dr0.4076.1.S1_at Endothelin 1 1.1 NC 1.3 1.1
1.6 0.5 1.0 1.0
Dr0.12454.1.S1_at GTP cyclohydrolase I feedback inhibitor 1.2 2.3 1.8 1.5
Dr0.6237.1.S1_at Kinesin family member C1 NC NC NC −1.9
Dr0.15087.1.A1_at Protein kinase C beta 1 NC NC NC −1.0
Notch signaling
Dr0.431.1.S1_at Hairy-related 6 1.1 NC NC NC
Dr0.25257.1.A1_at Similar to enhancer-of-split and hairy-related protein 1 NC 1.9 NC 1.5
Dr0.8232.1.S1_at Hairy/enhancer-of-split related with YRPW NC −1.2 NC NC
Motif 2 NC −1.2 NC NCc
TGF-β signaling
Dr0.8120.1.S1_at Noggin 1 NC 1.3 NC NC
Dr0.25506.1.A1_at Bmp receptor type 1a NC NC NC −1.3
Dr0.15495.1.A1_at Similar to bmp 6 precursor NC NC 1.4 1.1
Myogenesis and muscle-specific gene regulation
Dr0.25775.1.S1_at Iroquois homeobox protein 4a NC NC −1.1 NC
NC NC NC 0.7
Dr0.2499.1.S1_at Myogenin NC 1.2 1.7 2.9
NC NC NC 1.6
Dr0.8093.1.S1_at Paraxis NC 2.0 2.1 NC

  • NC, no significant change above 2-fold.

  • ↵ a GenBank accession number BI703179.

  • ↵ b Probe blasts to 3′-untranslated region of indicated gene.