Apigenin markedly inhibits the expression of costimulatory molecules (CD80, CD86, and CD40) and MHC class I and II molecules on LPS-stimulated CD11c + DC
BM-derived DC were cultured in the absence or presence of 20 μM apigenin following the 200 ng/ml LPS stimulation for 24 h. The expression of surface molecules was analyzed by FACSCalibur. Two-color flow cytometry was used to determine the level of antigen expression on CD11c + DC. The results are from one experiment of three performed.
Surface Antigen | Positive Cells (MFI) | ||||||
|---|---|---|---|---|---|---|---|
| Medium | Apigenin | LPS | Apigenin + LPS | ||||
| % | |||||||
| CD80 | 72 ± 3 (480 ± 25) | 73 ± 1 (372 ± 15)* | 87 ± 3 (1604 ± 86) | 75 ± 2 (642 ± 48)** | |||
| CD86 | 70 ± 4 (872 ± 42) | 68 ± 1 (510 ± 27) | 85 ± 1 (1853 ± 107) | 70 ± 3 (902 ± 86)** | |||
| MHC I | 71 ± 5 (392 ± 13) | 70 ± 2 (317 ± 8)* | 87 ± 3 (983 ± 103) | 74 ± 1 (562 ± 107)** | |||
| MHC II | 83 ± 4 (1250 ± 107) | 79 ± 1 (951 ± 102)* | 91 ± 2 (1802 ± 125) | 84 ± 4 (1379 ± 95)* | |||
| CD40 | 75 ± 3 (451 ± 12) | 73 ± 3 (370 ± 7)* | 86 ± 3 (1302 ± 97) | 77 ± 2 (570 ± 43)** | |||
>Statistical significance between samples with and without apigenin is indicated [*, p < 0.01 vs. unstimulated DC (medium); **, p < 0.01 vs. LPS-stimulated DC].