Plasmid Construct | Substituted Amino Acid | Charge at pH 6.0-7.0 | Charge Type/Mass | KD | KIH | KIL | % High-Affinity Sites | % Low-Affinity Sites |
---|---|---|---|---|---|---|---|---|
nM | nM | μM | ||||||
Wild type β1-AR | Lys | Basic (+) | Hydrophilic (polar)/146 | 19 ± 5 | 2 ± 0.14 | 0.35 ± 0.02 | 44 ± 6 | 54 |
K321E | Glu | Acidic (−) | Hydrophilic (polar)/174 | 29 ± 8 | 4.2 ± 0.3 | 0.35 ± 0.03 | 42 ± 5 | 58 |
K321M | Met | Neutral | Hydrophobic nonpolar/149 | 24 ± 6 | 4.7 ± 0.4 | 0.7 ± 0.07 | 45 ± 8 | 56 |
K321A | Ala | Neutral | Hydrophobic nonpolar/89 | 21 ± 4 | 5.3 ± 0.4 | 0.5 ± 0.04 | 46 ± 5 | 54 |
K324E | Glu | Acidic (−) | Hydrophilic (polar)/174 | 21.4 ± 4 | 0.87 ± 0.03 | 100 | ||
K324M | Met | Neutral | Hydrophobic nonpolar/149 | 21.6 ± 4 | 1.1 ± 0.2 | 100 | ||
K324A | Ala | Neutral | Hydrophobic nonpolar/89 | 28.6 ± 7 |
| 1.3 ± 0.4 |
| 100 |