TABLE 1

Inhibition of the catalytic activity of VC1:IIC2 by various MANT-nucleotides and TNP-nucleotides

The catalytic activity of VC1:IIC2 was determined in the presence of 10 mM MnCl₂ and 100 μM FSK in the absence or presence of GTPγS-activated Gα_s as described under Materials and Methods. Reaction mixtures contained 40 μM ATP/Mn²⁺, 1 μCi of [α-³²P]ATP, and TNP or MANT nucleotides at increasing concentrations to obtain saturated inhibition curves. K_i values were calculated from IC₅₀ values using the previously published K_m values (Mou et al., 2005). Data shown are the means ± S.D. of three to six independent experiments performed in duplicate.