TABLE 1

ANOVA results for effects of nAChR gene, genotype, and brain region on binding sites for [³H]epibatidine, [³H]cytisine, and ¹²⁵I-αBgt

F values for the main effects of gene (α7, β2, or β4), genotype (+/+,+/− or −/−) and brain region (thalamus, hippocampus, superior colliculus, or inferior colliculus) on each of the six binding sites are presented. All results were initially analyzed by three-way ANOVA and subsequently analyzed for each gene by two-way ANOVA. Results of subsequent one-way ANOVA are presented in the figure legends.

	df	[³H]Cytisine Bound	Cytisine-Sensitive [³H]Epibatidine Bound	Cytisine-Resistant [³H]Epibatidine Bound	¹²⁵I-αBgt Bound	αBgt-Sensitive [³H]Epibatidine Bound	αBgt-Resistant [³H]Epibatidine Bound
Three-way ANOVAs
Main effects
Receptor gene	2	119.62^***	97.85^***	37.86^***	165.56^***	37.38^***	56.98^***
Genotype	2	24.47^***	42.60^***	26.93^***	89.10^***	7.50^***	20.99^***
Brain region	3	171.41^***	205.06^***	186.75^***	210.31^***	29.59^***	92.45^***
Two-way interactions
Gene by genotype	4	37.34^***	31.22^***	12.47^***	65.40^***	8.96^***	3.93^**
Gene by brain region	6	8.45^***	17.05^***	8.70^***	12.38^***	5.67^***	17.06^***
Genotype by brain region	6	1.61	3.11^**	1.88	4.40^***	0.73	1.70
Three-way interaction
Gene by genotype by brain region	12	2.79^**	2.85^**	3.76^***	4.04^***	0.70	1.50
Residual df		107	192	192	132	237	237
Two-way ANOVAs
α7 Subunit
Main effects
Genotype	2	0.76	1.00	0.05	331.49^***	18.05^***	2.12
Brain region	3	97.16^***	106.23^***	53.23^***	58.32^***	1.10	43.62^***
Two-way interaction
Genotype by brain region	6	0.83	0.31	0.30	17.47^***	1.36	0.25
Residual df		35	59	59	60	72	72
β2 Subunit
Main effects
Genotype	2	282.86^***	110.01^***	55.27^***	0.04	0.90	39.88^***
Brain region	3	52.72^***	39.99^***	48.59^***	79.92^***	32.07^***	13.91^***
Two-way interaction
Genotype by brain region	6	15.63^***	8.33^***	8.86^***	0.15	0.17	5.82^***
Residual df		36	71	71	36	104	104
β4 Subunit
Main effects
Genotype	2	0.16	0.54	10.38^***	0.08	0.04	0.76
Brain region	3	48.28^***	98.22^***	124.56^***	74.09^***	16.95^***	43.99^***
Two-way interaction
Genotype by brain region	6	0.50	0.25	2.73^*	0.53	0.12	0.40
Residual df		36	62	62	36	61	61

^*, P < 0.05; ^**, P < 0.01; ^***, P < 0.001.