TABLE 1
Quantification of cell-permeable peptides inside HPAECs
Cell volume was determined by incubating HPAECs with [3H]mannitol as described under Materials and Methods. A volume of 2.5 μl/106 cells was obtained. Cells were incubated with medium containing the indicated concentrations of peptides in a total volume of 2.46 ml/106 cells. The relative concentrations of peptides present in the cell extracts were calculated using mass spectra from known peptide concentrations and cell volume (see details under Materials and Methods). Values reported are mean of three separate experiments.