Immunoprecipitation of native [3H]epibatidine-labeled human AChRs by mAbs to α6 subunits
The cell lines expressing α3β2 and α4β2 AChRs were described previously (Wang et al., 1998; Kuryatov et al., 2005). A 0.5 nM concentration of AChRs and 1.5 nM [3H]epibatidine were used in these assays using triplicate samples.