TABLE 1

Immunoprecipitation of native [3H]epibatidine-labeled human AChRs by mAbs to α6 subunits

The cell lines expressing α3β2 and α4β2 AChRs were described previously (Wang et al., 1998; Kuryatov et al., 2005). A 0.5 nM concentration of AChRs and 1.5 nM [3H]epibatidine were used in these assays using triplicate samples.


AChR

Titer of mAb
mAb 338
mAb 339
mAb 349
mAb 350
mAb 351
mAb 353
μmol [3H]epibatidine binding sites/l mAb
α3β2 0 0 0 0 0 0
α4β2 0 0 0 0 0 0
α6β2
4.1
0.09
0.3
5.7
6
0