Parameters for activation and inactivation kinetics of Nav1.5 N405 channel mutants
The voltage half-points of activation and inactivation (V½) and slope factors (dx) were derived from Boltzmann fits (as described in Figure 7). The uncharged substitutions (Ala, Cys, Phe) were made in the Nav1.5 background (WT), whereas the charged substitutions (Asp, Glu, Arg, Lys) were performed in the Nav1.5 C373Y (WT-C373Y) channel.
Channel | Activation | Inactivation | ||||
|---|---|---|---|---|---|---|
| V½ (n) | dx | V½ (n) | dx | |||
| mV | ||||||
| WT | −47.6 ± 1.4 (19) | 7.6 ± 0.5 | −94.2 ± 1.5 (19) | 5.4 ± 0.1 | ||
| WT-C373Y | −46.7 ± 1.0 (9) | 6.6 ± 0.1 | −91.7 ± 1.5 (9) | 5.3 ± 0.3 | ||
| N406A | −24.8 ± 1.1 (19) | 9.3 ± 0.2 | −84.5 ± 1.0 (19) | 5.7 ± 0.1 | ||
| N406C | −44.1 ± 1.0 (13) | 7.0 ± 0.3 | −99.0 ± 1.0 (13) | 6.0 ± 0.2 | ||
| N406F | −31.8 ± 0.6 (12) | 8.2 ± 0.2 | −100.5 ± 1.3 (12) | 5.7 ± 0.2 | ||
| N406D | −35.2 ± 0.9 (16) | 7.2 ± 0.1 | −94.9 ± 1.3 (16) | 4.9 ± 0.2 | ||
| N406E | −55.4 ± 1.9 (8) | 6.8 ± 0.3 | −90.6 ± 2.2 (8) | 5.5 ± 0.5 | ||
| N406R | −46.8 ± 1.5 (9) | 6.4 ± 0.2 | −90.8 ± 1.9 (9) | 4.9 ± 0.4 | ||
| N406K | −48.2 ± 1.3 (16) | 6.7 ± 0.1 | −88.4 ± 1.5 (16) | 4.1 ± 0.3 | ||