Sequences of primer pairs and Taqman probes
Human COX-2 |
|
| Probe | 5′-Quasar670d (AGCCCTTCCTCCTGTGCCTGATGA) BHQ-2-3′ |
| Forward | 5′-GAAGCCTTCTCTAACCTCTCCTAT-3′ |
| Reverse | 5′-TCCCACAATCTCATTTGAATCAGG-3′ |
| Human iNOS | |
| Probe | 5′-Quasar670d (TCCGACATCCAGCCGTGCCACCA) BHQ-2-3′ |
| Forward | 5′-AGGCTCAAATCTCGGCAGAATC-3′ |
| Reverse | 5′-CCTCACAGGAGAGTTCCACCA-3′ |
| Mouse COX-2 | |
| Probe | 5′-Quasar670d (CCCTGCTGCCCGACACCTTCAACA) BHQ-2-3′ |
| Forward | 5′-TTCAACACACTCTATCACTGGCAC-3′ |
| Reverse | 5′-GCAATCTGTCTGGTGAATGACTCA-3′ |
| Mouse iNOS | |
| Probe | 5′-Quasar670d (CCGCAGCTCCTCACTGGGACAGCA) BHQ-2-3′ |
| Forward | 5′-CCCTAAGAGTCACCAAAATGGCTC-3′ |
| Reverse | 5′-ATACTGTGGACGGGTCGATGG-3′ |
| β-Actin | |
| Probe | 5′-T (CalRed) d (CAAGATCATGTCTCCTCCTGAGCGCA) BHQ-2-3′ |
| Forward | 5′-ATTGCCGACAGGATGCAGAA-3′ |
| Reverse | 5′-GCTGATCCACATCTGCTGGAA-3′ |