TABLE 1
Binding characteristics of the μ-opioid receptor with and without coexpression of synaptophysin
KD and Bmax for the binding of [3H] DAMGO to μ-opioid receptors in stable cell line expressing HA epitope-tagged MOPr with or without coexpression of Myc epitopetagged Syp in HEK293 cells were determined by Scatchard analyses. The effect of 5 μM DAMGO on forskolin-stimulated cAMP accumulation was determined as described under Materials and Methods. Values shown are the mean ± S.E.M. of three independent experiments performed in duplicate.