TABLE 2

Cisplatin-sensitive deletion strains

Mutants showing a significant growth defect or absence of growth after 1 day in the presence of 250 μg/ml cisplatin scored as “×” or “× ×.” Mutants showing a significant growth defect or absence of growth after 3 days in the presence of 250 μg/ml cisplatin scored as “× × ×” or “× × × ×.”

ORF
Gene
Sensitivity
Cellular Function and Comments
DNA replication and damage repair
YJL092W HPR5 × × × × DNA helicase involved in DNA repair
YMR137C PSO2 × × × × DNA cross-link repair protein
YPL022W RAD1 × × × × Single-stranded DNA endonuclease involved in DNA repair
YGR258C RAD2 × × × × Single-stranded DNA endonuclease involved in DNA repair
YER162C RAD4 × × × × NER
YML095C RAD10 × × × × Single-stranded DNA endonuclease involved in DNA repair
YMR201C RAD14 × × × × NER
YCR066W RAD18 × × × × DNA PR
YNL250W RAD50 × × × × Subunit of MRX complex involved in double-strand break repair
YER095W RAD51 × × × × DNA recombinational repair
YML032C RAD52 × × × × DNA double-strand break repair
YDR076W RAD55 × × × × DNA recombinational repair
YDR004W RAD57 × × × × DNA recombinational repair
YLR234W TOP3 × × × × DNA topoisomerase III
YDR369C XRS2 × × × × DNA recombinational repair
YLR235C × × × × ORF overlap with TOP3
YPR135W CTF4 × × × × Required for sister chromatid cohesion
YIL154C IMP2' × × × × Transcriptional activator involved in protection against DNA damage
YBR098W MMS4 × × × DNA endonuclease
YDR386W MUS81 × × × DNA repair and replication fork stability
YJR043C POL32 × × × Polymerase-associated gene
YLR032W RAD5 × × × Single-stranded DNA-dependent ATPase involved in PR
YDL059C RAD59 × × × Involved in double-strand break repair
YIL139C REV7 × × × Subunit of DNA polymerase ζ involved in DNA repair
YJL115W ASF1 × × Nucleosome assembly factor
YDL013W HEX3 × × Involved in the DNA Damage response with possible recombination role
YGL087C MMS2 × × DNA PR
YGL175C SAE2 × × Involved in meiotic recombination and chromosome metabolism
YMR190C SGS1 × × Nucleolar DNA helicase of the RecQ family
YLR135W SLX4 × × DNA repair and replication fork stability
YGL168W HUR1 × Required for hydroxyurea resistance and DNA replication
YCL060C MRC1 × Required for DNA replication and RaD53 checkpoints activation
YGL167C PMR1 × ORF overlap with HUR1
Vacuolar V-ATPase and vacuolar function
YPR036W VMA13 × × × Vacuolar ATPase V1 domain subunit
YOR332W VMA4 × × Vacuolar ATPase V1 domain subunit
YLR447C VMA6 × × Vacuolar ATPase V0 domain subunit
YGR020C VMA7 × × Vacuolar ATPase V1 domain subunit
YEL051W VMA8 × × Vacuolar ATPase V1 domain subunit
YCL007C VMA9 × × Vacuolar ATPase V0 domain subunit
YHR039C-B VMA10 × × Vacuolar ATPase V0 domain subunit
YHR026W VMA16 × × Vacuolar ATPase V0 domain subunit
YKL119C VPH2 × × Required for the biogenesis of a functional vacuolar ATPase
YLR396C VPS33 × × Vacuolar sorting protein essential for vacuolar morphogenesis/function
YOR331C × × ORF overlap with VAM4
YKL118W × × ORF overlap with VPH2
Cell cycle
YLR337C VRP1 × × × Involved in cytoskeletal organization and cellular growth
YCR063W BUD31 × × Involved in bud-site selection
YLR226W BUR2 × × Bypass UAS requirement
YHR191C CTF8 × × Required for sister chromatid cohesion
YMR078C CTF18 × × Required for sister chromatid cohesion
YCL016C DCC1 × × Required for sister chromatid cohesion
YGL240W DOC1 × × Required for the activity of APC
YPR119W CLB2 × Involved in mitotic induction
YGR092W DBF2 × Kinase required for late nuclear Division
YGR252W GCN5 × Histone acetyltransferase, acetylates lysine 14 on histone H3
Cell stress and signal transduction
YNL064C YDJ1 × × × × Heat shock protein
YDR075W PPH3 × × × Catalytic subunit of protein phosphatase 2A
YJR104C SOD1 × × × Cu,Zn superoxide dismutase
YGR285C ZUO1 × × × Cytosolic ribosome-associated chaperone
YLR403W SFP1 × × Transcription factor controls expression of ribosome biogenesis genes (in response to nutrients and stress)
YJL165C HAL5 × Putative protein kinase involved in sodium and lithium tolerance
Ribosomal protein and protein synthesis
YKL204W EAP1 × × eIF4E-associated protein
YGL143C MRF1 × × Mitochondrial polypeptide chain release factor
YMR142C RPL13B × × Component of the 60S ribosomal subunit
YGL076C RPL7A × Component of the 60S ribosomal subunit
YCL037C
SRO9
×
RNA-binding protein which associates with translating ribosomes
Transcription
YGL070C RPB9 × × RNA polymerase II core subunit
YCR065W HCM1 × Transcription factor involved in cell cycle specific transcription
Transporter
YJL129C TRK1 × × 180-kDa high-affinity potassium transporter
YGL084C GUP1 × Membrane protein and putative glycerol transporter
Others
YKR082W NUP133 × × × Subunit of f the nuclear pore complex
YNL280C ERG24 × × C-14 sterol reductase
YGL076C FYV5 × × Unknown
YLR376C PSY3 × × Unknown; deletion results in sensitivity to oxaliplatin & cisplatin
YJR121W ATP2 × F1 subunit of mitochondrial F1F0 ATP synthase
YDR245W
MNN10
×
Subunit of a Golgi mannosyltransferase complex

  • ORF, open reading frame; MRX, Mrell-Radso-Xrs2; UAS, upstream activation sequence; APC, anaphase promoting complex.