The summary of single-channel kinetic analysis under control conditions and in the presence of combinations of ethanol and 3α5αP for the receptor containing a γ subunit where the carboxylterminal sequence was replaced with homologous sequence from the ρ subunit (γ −ρ subunit).
The mean durations (OT1-3, CT1, CT2, and CTβ) and relative contributions (fraction OT1-3, fraction CT1, CT2, and CTβ) for the three open and closed time components are shown. All data were obtained in the presence of 50 μM GABA to activate the channel. Statistical analysis was carried out using ANOVA with Bonferroni correction (Systat 7.0; Systat Software). Only the fraction of OT3 for 1 μM 3α5αP reached significant (P < 0.05) difference from control (no steroid/no ethanol) conditions. All other parameters examined (mean duration OT3, fraction OT3, fraction CTβ) at all other conditions studied showed nonsignificant effects.
3α5αP | Ethanol | OT1 | Fraction OT1 | OT2 | Fraction OT2 | OT3 | Fraction OT3 | CT1 | Fraction CT1 | CT2 | Fraction CT2 | CTβ | Fraction CTβ | n |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ms | ms | ms | ms | ms | ms | |||||||||
| None | None | 0.34 ± 0.12 | 0.24 ± 0.10 | 3.5 ± 0.8 | 0.54 ± 0.20 | 6.9 ± 1.8 | 0.22 ± 0.16 | 0.14 ± 0.02 | 0.51 ± 0.07 | 1.3 ± 0.4 | 0.21 ± 0.09 | 12.3 ± 4.0 | 0.27 ± 0.07 | 5 |
| None | 0.1% | 0.29 ± 0.07 | 0.20 ± 0.02 | 3.0 ± 0.5 | 0.50 ± 0.17 | 6.9 ± 0.8 | 0.30 ± 0.17 | 0.15 ± 0.01 | 0.56 ± 0.03 | 1.7 ± 1.1 | 0.17 ± 0.06 | 12.1 ± 1.0 | 0.27 ± 0.05 | 3 |
| 100 nM | None | 0.23 ± 0.09 | 0.19 ± 0.06 | 3.4 ± 1.4 | 0.40 ± 0.32 | 7.9 ± 1.8 | 0.41 ± 0.26 | 0.18 ± 0.04 | 0.30 ± 0.16 | 1.4 ± 0.2 | 0.27 ± 0.03 | 11.6 ± 3.1 | 0.43 ± 0.13 | 4 |
| 1 μM | None | 0.30 ± 0.09 | 0.22 ± 0.10 | 3.0 ± 1.8 | 0.24 ± 0.13 | 10.6 ± 2.8 | 0.54 ± 0.14 | 0.17 ± 0.07 | 0.44 ± 0.14 | 1.7 ± 0.4 | 0.36 ± 0.06 | 12.6 ± 4.1 | 0.20 ± 0.13 | 10 |
| 100 nM | 0.1% | 0.24 ± 0.11 | 0.24 ± 0.09 | 3.1 ± 1.7 | 0.33 ± 0.15 | 10.1 ± 4.9 | 0.43 ± 0.16 | 0.16 ± 0.04 | 0.38 ± 0.16 | 1.5 ± 0.1 | 0.27 ± 0.05 | 12.6 ± 6.4 | 0.35 ± 0.14 | 8 |
| 1 μM | 0.1% | 0.45 ± 0.15 | 0.17 ± 0.01 | 4.5 ± 1.9 | 0.53 ± 0.35 | 10.6 ± 0.9 | 0.31 ± 0.34 | 0.16 ± 0.03 | 0.34 ± 0.22 | 1.6 ± 0.1 | 0.32 ± 0.05 | 14.4 ± 9.8 | 0.34 ± 0.17 | 2 |