PCR primers for amplification of the human CHRNA7 gene
Primer Sequence | PCR Product Size | |
|---|---|---|
| bp | ||
| Promoter | ||
| Sense | 5′-CAGAATTGTCCCGGCTTTCTCCCG-3′ | 320 |
| Antisense | 5′-GCCTCTCCACGTGACGAGCCCC-3′ | |
| Promoter + exon 1 | ||
| Sense | 5′-GTTCCCTGGGTGGCCGCCGAGA-3′ | 335 |
| Antisense | 5′-TCCCACGGAGGAGTGGAGGGCG-3′ | |
| Exon 2 | ||
| Sense | 5′-CCCCCTGCCCGGGTCTTCTCTCCTTA-3′ | 237 |
| Antisense | 5′-ACGCTGTCTAAAAAGCCCCTCGGAGC-3′ | |
| Exon 3 | ||
| Sense | 5′-CACACACAACAACGCTCTCGACAG-3′ | 207 |
| Antisense | 5′-TCCTAAACAGGACCTCTCAGAAGCAA-3′ | |
| Exon 4 | ||
| Sense | 5′-CAAGGAAGTGAAGTGCTGCTAATGTC-3′ | 211 |
| Antisense | 5′-ACGTGATAGCTACATGTATGCAAGCA-3′ | |
| Exon 5 to exon 10a | ||
| Sense | 5′-CTGCAGTTCAGTCATTCAAGATATATATGTGGG-3′ | 17,367 |
| Antisense | 5′-CACTTCTACTTGTTTCTAAAGACACTGTAACAG-3′ | |
| Exon 5 to exon 10a | ||
| Sense | 5′-CCACTTCCAGTGAGGTTTTCTCTGAC-3′ | 16,480 |
| Antisense | 5′-CAGGCAGTGGGCTAATGGGCAAAAT-3′ | |
| Exon 5 | ||
| Sense | 5′-GGTCTTTGCTGCTCCATCAAAGACAG-3′ | 238 |
| Antisense | 5′-ACTGACTGACACCCAAACTCGCTTCA-3′ | |
| Exon 6 | ||
| Sense | 5′-CAGCACATCTCAGTCAGCTTCCGTTT-3′ | 265 |
| Antisense | 5′-GAATAGGAAAGCTTTCTTCCAGGCGG-3′ | |
| Exon 7 | ||
| Sense | 5′-AACAATGGCTCCTTCTCTCCTCCTCC-3′ | 279 |
| Antisense | 5′-CAGCAGAAGGTCTCCAGTCTCAGACT-3′ | |
| Exon 8 | ||
| Sense | 5′-AGAGAGCCCTCGTTAGACAGAATTGA-3′ | 226 |
| Antisense | 5′-AAATCCTGGGCACACTCTAACCCTAA-3′ | |
| Exon 9 | ||
| Sense | 5′-CAGAGAACCTGATCAGGGTGTGCCT-3′ | 355 |
| Antisense | 5′-TGGGTGACATAGTGAGACTCCGTCT-3′ | |
| Exon 10-1 | ||
| Sense | 5′-CCGCCTCAGGGCTGCTCTTAACG-3′ | 316 |
| Antisense | 5′-GTGCAGGAGGTGCTCATCGTGCG-3′ | |
| Exon 10-2 | ||
| Sense | 5′-GACTCTGGGGTAGTGTGTGGCCG-3′ | 365 |
| Antisense | 5′-CGCCAAGCCAAAGGCCTTGCCCA-3′ | |
| Exon 10-3 | ||
| Sense | 5′-GGCCGTGTCCAAAGACTTTGCGTAA-3′ | 306 |
| Antisense | 5′-CAGGCAGTGGGCTAATGGGCAAAAT-3′ | |
| Exon 10-4 | ||
| Sense | 5′-TATCCTTGGCACATCCGTACCAT-3′ | 353 |
| Antisense | 5′-CATCAAGCTGTTTCTCTCTACCGTC-3′ | |
| Exon 10-5 | ||
| Sense | 5′-CTCTTAGCTTTTCTGCAATTCAAC-3′ | 357 |
| Antisense | 5′-TGGGCTGTTAATTCTAGTAGAACTC-3′ |
|
↵ a Primers used in primary PCR.