Effect of pioglitazone on the activities of P450c17 and 3βHSDII
Enzyme activities were assessed by calculating the substrate to product conversion ratios from data shown in Fig. 1. Data are expressed as the percentage of control and represent the mean of three independent experiments ± S.D.
Enzyme Activities | ||||||||
|---|---|---|---|---|---|---|---|---|
| Basal | cAMP-Stimulated | |||||||
| DMSO | 10 μM PIO | DMSO | 10 μM PIO | |||||
| % | ||||||||
| 17OH Preg/Preg | 17α-Hydroxylase | 100 | 109 ± 15 | 100 | 63 ± 7 | |||
| DHEA/17OH Preg | 17,20-Lyase | 100 | 63 ± 6 | 100 | 81 ± 5 | |||
| 17OH Prog/17OH Preg | 3βHSDII | 100 | 58 ± 7.5 | 100 | 64 ± 5 | |||
| A/DHEA | 3βHSDII | 100 | 63 ± 15 | 100 | 76 ± 12 | |||
A, androstenedione; Preg, pregnenolone; 17OH Prog, 17α-hydroxyprogesterone; 17OH Preg, 17α-hydroxypregnenolone.