In vitro nicotine metabolism (C-oxidation) parameters
DBA/2 (n = 5 mice) | C57BL/6 (n = 4 mice) | ||
|---|---|---|---|
| mean ± S.D. | |||
| High affinity | |||
| Km (μM) | 10.7 ± 4.8 | 11.4 ± 3.6 | |
| Vmax (nmol/min/mg) | 0.58 ± 0.18 | 0.50 ± 0.07 | |
| Vmax/Km | 0.05 ± 0.03 | 0.04 ± 0.02 | |
| Low affinity | |||
| Km (μM) | 234 ± 77 | 306 ± 126 | |
| Vmax (nmol/min/mg) | 1.60 ± 0.63 | 1.18 ± 0.36 | |
| Vmax/Km | 0.007 ± 0.001* | 0.004 ± 0.001 | |
↵* p < 0.05 compared with C57BL/6 mice.