TABLE 2

RGS DNA plasmid dose dependency for inhibition of mAChR InsP_x signals

HEK293T cells were transiently co-transfected in 24-well plates with 50 ng/well of the M₁, M₃, or M₅ mAChR and increasing amounts of RGS2 or RGS8 plasmid DNA. Whole-cell accumulation of [³H]InsP_x was measured with 200 nM carbachol, and the IC₅₀ values were calculated. Cheng-Prusoff corrected IC₅₀ values were calculated from the equation: Corrected IC₅₀ = IC₅₀/[1 + ([carbachol]/EC₅₀)]. Results are the mean ± S.E. of three individual experiments performed in triplicate.