Summary of Km and Vmax values determined from substrate velocity analysis as described under Materials and Methods
Values are the mean ± S.D. of three experiments performed in duplicate.
Km | Vmax | |
|---|---|---|
| μM | nmol phosphate/min | |
| PP2A | 242 ± 109 | 1.7 ± 0.3 |
| PP2A + GST | 337 ± 246 | 3.3 ± 1.2 |
| PP2A + Gα12 | 123 ± 25 | 11.0 ± 0.6 |