Sequences of Q-MSP primers
Primer Name | Q-MSP Primer Sequence (5′→3′) |
|---|---|
| PKCϵAP-1 | |
| FM | gatgtgtttaagggttgac |
| RM | ctacacacgaaaatccg |
| FUM | gatgtgtttaagggttgat |
| RUM | cctacacacaaaaatcca |
| PKCϵCREB | |
| FM | tgttaataatgttcggttttatac |
| RM | cgtaataatataaaataacactcacg |
| FUM | gtatgttaataatgtttggttttatat |
| RUM | acataataatataaaataacactcaca |
| PKCϵCREB/cJun1 | |
| FM | tatgttaataatgttcggttttatac |
| RM | cgtataaaattctcaaaaatattaacg |
| FUM | gtatgttaataatgtttggttttatat |
| RUM | acatataaaattctcaaaaatattaaca |
| PKCϵCREB/cJun2 | |
| FM | ggtgaaaataaatatagttgattttatc |
| RM | atttcctacgaatacccg |
| FUM | ggtgaaaataaatatagttgattttatt |
| RUM | cttaatttcctacaaataccca |
| PKCδAP-1 | |
| FM | ttttatgcgtttgattgatcgagc |
| RM | ccgccgccaaacctaccg |
| FUM | ttttatgtgtttgattgattgagt |
| RUM | ccaccaccaaacctacca |
| PKCδNFκB | |
| FM | gcgtaagtagttggggaagtttcgtc |
| RM | acctcgcctaccaataacacg |
| FUM | gtgtaagtagttggggaagttttgtt |
| RUM | acctcacctaccaataacaca |
| PKCδEgr-1 | |
| FM | tttcgtgggcggagtttcgaaggggc |
| RM | cccaaataccgaccccgatcccg |
| FUM | ttttgtgggtggagttttgaaggggt |
| RUM | cacccaaataccaaccccaatccca |
| GAPDH | |
| FM | tttggttattagggttgttttttt |
| RM | taataaccaacttcccattctc |
| FUM | cctggttaccagggctgc |
| RUM | gggtagagtcatactggaacatg |
FM, forward orientation, methylated; RM, reverse orientation, methylated; FUM, forward orientation, unmethylated; RUM, reverse orientation, methylated.