293B sensitivity of mutant KCNQ1 channels
IC50 values are expressed as mean ± S.E.M. n indicates number of experiments.
Mutation in KCNQ1 | KCNQ1 | KCNQ1/KCNE1 (IKs) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IC50 | Inhibition at 100 μM 293B | n | IC50 | Inhibition at 100 μM 293B | n | |||||
| μM | % | μM | % | |||||||
| KCNQ1 wild type | 26.9 ± 0.8 | 5 | 6.9 ± 0.5 | 5 | ||||||
| V307L | 48.7 ± 3.2 | 4 | 49.9 ± 2.2 | 4 | ||||||
| V310L | 70.5 ± 2.1 | 3 | 30.5 ± 1.1 | 3 | ||||||
| T311S | 30.1 ± 1.7 | 3 | 7.2 ± 0.7 | 3 | ||||||
| T311A | No functional expression | Not tested | ||||||||
| T312S | ∼10 | 4 | Not tested | |||||||
| K326R/T327L | 29.5 ± 1.1 | 3 | Not tested | |||||||
| S330A/C331T | 32.4 ± 2.0 | 3 | Not tested | |||||||
| F335Y | ∼50 | 3 | 9.1 ± 2.5 | 3 | ||||||
| A336T | ∼40 | 3 | ∼50 | 3 | ||||||
| I337V | ∼35 | 4 | ∼39 | 4 | ||||||
| S338T | ∼40 | 4 | 10.2 ± 3.7 | 4 | ||||||
| F339Y | ∼40 | 3 | 20.2 ± 1.7 | 3 | ||||||
| F340I | ∼20 | 3 | Not tested | |||||||
| F340Y |
| ∼10 | 4 |
| ∼30 | 4 | ||||