TABLE 1

Gene-specific primers used in RT-PCR

Gene	GenBank Accession Number	Direction	Oligonucleotide Sequence [5′→3′]	Product Size	Melting Temperature
				bp	°C
β-Actin^a	V01217	Forward	TTTGAGACCTTCAACACCCCAGCCAT	266	88
		Reverse	ATGTCACGCACGATTTCCCTCTCA
CaMKII	NM_012920	Forward	TGGAGGCTGTGCTACACTGTCACCAG	314	89
		Reverse	CTGCTGGTACAGGCGGTGCTGGTC
GAD65	M72422	Forward	TGGGTGTCCCCTTGCAATGTTCGG	203	89
		Reverse	AGTAGTCCCCTTTGCTCTCCACATGA
GFAP	L27219	Forward	TCCTTCTGTTTTTATGCCCACGGC	308	87
		Reverse	ATGTTTTCCTTTCTGTCT
α_2AAR	NM_012739	Forward	CGTGGTGTGTTGGTTCCCGTTCTTTT	180	89
		Reverse	CGGCAGAGGATCTTCTTGAAGGC
α_2BAR	NM_138505	Forward	AGAGGAGGGAGATGAAGAGGATGAGG	399	89
		Reverse	AAGACGGTGTAGATGACAGGGTTCAAA
α_2CAR	NM_138506	Forward	TTCGTACTGTGCTGGTTCCCCTTCTTCTTC	196	89
		Reverse	TCCTACGGAAGAGGATGTGCTTGAAAG

↵ a Housekeeping gene. The genomic primer recognized the polymorphic repeat STS ET3 (forward, GCCTGCATTCATCTTCATCTGC; reverse, AAAGGTGGAACTCGCCCGTTT).