Influence of hypertonic stimulation on gene expression of drug-metabolizing enzymes in human primary hepatocytes (cDNA microarray analysis)
Criteria: change >2- or <0.5-fold in response to both NaCl (+20 or +50) and sucrose (Suc50 or Suc100) exposure compared with isotonic medium in both lines of primary hepatocyte culture (line 1 and line 2).
Gene | Line 1 | Line 2 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NaCl + 20 | NaCl + 50 | Suc50 | Suc100 | NaCl + 20 | NaCl + 50 | Suc50 | Suc100 | |||||||
| -fold change | ||||||||||||||
| CYP1A1 | 1.4 | 4.3 | 1.7 | 3.2 | 2.5 | 2.0 | 3.1 | 1.3 | ||||||
| CYP1A2 | 0.3 | 2.0 | 0.1 | 0.9 | 1.4 | 0.2 | 0.7 | 0.5 | ||||||
| CYP2E1 | 1.3 | 3.2 | 0.6 | 2.4 | 6.0 | 6.6 | 8.5 | 3.5 | ||||||
| UGT2B4 | 1.7 | 2.5 | 3.3 | 2.3 | 1.7 | 3.0 | 2.6 | 2.7 | ||||||