Binding properties of cysteine-substituted mutant hAT1 receptors
Cells transfected with the appropriate receptor were assayed as described under Materials and Methods. Binding affinities (Kd) and maximal binding capacities (Bmax) are expressed as the means ± S.D. of values obtained in n independent experiments performed in duplicate. Mutants D263C and P255C demonstrated no detectable binding.
Kd | Bmax | n | |
|---|---|---|---|
| nM | fmol/mg | ||
| WT | 1.1 ± 0.4 | 1643 ± 1075 | 6 |
| L265C | 0.6 ± 0.2 | 1233 ± 457 | 3 |
| V264C | 0.8 ± 0.2 | 983 ± 329 | 3 |
| L262C | 1.1 ± 0.4 | 2630 ± 1151 | 3 |
| F261C | 1.0 ± 0.8 | 923 ± 587 | 3 |
| T260C | 1.1 ± 0.1 | 780 ± 79 | 3 |
| F259C | 0.8 ± 0.3 | 1553 ± 46 | 3 |
| I258C | 0.8 ± 0.2 | 2457 ± 284 | 3 |
| Q257C | 3.4 ± 1.3 | 903 ± 252 | 3 |
| H256C | 1.1 ± 0.2 | 1967 ± 72 | 3 |
| I254C | 0.7 ± 0.2 | 1657 ± 520 | 3 |
| W253C | 1.4 ± 0.3 | 1120 ± 825 | 3 |
| S252C | 1.0 ± 0.5 | 1217 ± 878 | 3 |
| F251C | 0.8 ± 0.5 | 1100 ± 377 | 3 |
| F250C | 1.0 ± 0.3 | 1317 ± 488 | 3 |
| F249C | 1.6 ± 0.3 | 837 ± 205 | 3 |
| F248C | 1.0 ± 0.3 | 767 ± 238 | 3 |
| L247C | 0.8 ± 0.2 | 620 ± 365 | 3 |
| V246C | 0.9 ± 0.1 | 577 ± 248 | 3 |
| I245C | 0.9 ± 0.2 | 1503 ± 540 | 3 |
| A244C | 0.6 ± 0.2 | 790 ± 516 | 3 |
| M243C | 0.7 ± 0.2 | 1200 ± 576 | 3 |
| I242C | 0.5 ± 0.1 | 1293 ± 539 | 3 |
| I241C | 0.7 ± 0.2 | 1240 ± 519 | 3 |
| K240C | 0.7 ± 0.1 | 1440 ± 357 | 3 |