Primer sequences used to generate the CYP3A4 nested deletion and mutation reporter plasmids
Residues that match wild-type sequences are shown in upper case. Mutagenized residues are shown in lower case.
Nested deletions |
|
| -2795 | 5′-gctagcAGGGGAGAGGCATG-3′ |
| -301 | 5′-gctagcGAGACAAGGGCAAG-3′ |
| -222 | 5′-gctagcCCCTTGGACTCCCCA-3′ |
| -170 | 5′-gctagcTATGAACTCAAAGG-3′ |
| -150 | 5′-gctagcGTGAGTGGTGTGTG-3′ |
| -80 | 5′-gctagcCAGGTGGCCCTGC-3′ |
| -35 | 5′-gctagcCTAGCATATAAACA-3′ |
| -24 | 5′-gctagcACAATCCAACAGC-3′ |
| Reverse | 5′-aagcttTGGGATGAGAGCCAT-3′ |
| Mutations | |
| 3A4-57 bp-KO | |
| Forward | 5′-/5Phos/-TGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTAGC-3′ |
| Reverse | 5′-/5Phos/-GTTGGCAAAGAATCACACACACACCACTCACTGACCTCC-3′ |
| 3A4-SacII | |
| Forward | 5′-CAGGCAGAGCACAGGTGcCgCgGCTACTGGCTGCAGCTCCAGC-3′ |
| Reverse | 5′-GCTTCTCCACCgcGGAAGTTGGCAAAGAATCACAC-3′ |
| 3A4CAATSpacer | |
| Forward | 5′-acgaacgaacgaacgaacgaacgaTGCTACTGGCTGCAGCTCCAGCCCTGCCTCCTTCTCTAGC3′ |
| Reverse | 5′-cgttcgttcgttcgttcgttcgGGAAGTTGGCAAAGAATCACACACACACCACTCACTGACCTCC3′ |
KO, knockout.