TABLE 1

Adenovirus infection of cardiac myocytes and maintenance of rod-shaped morphology

Number of rod-shaped cardiac myocytes 48 h after infection with AdLacZ, AdGRK2, AdGRK3, AdGRK2ct, or AdGRK3ct. For all recombinant adenoviruses, infection was performed at same m.o.i. (500). The viral titers of all recombinant adenoviruses were determined simultaneously in cells seeded from same preparation of isolated cardiac myocytes. Same number of cells were seeded in cell culture dishes (100,000 per 35-mm well), infected 2 h after plating, and assayed 48 h after infection. Data represent number of rod-shaped myocytes per dish (mean ± S.E.M.; n = 6 dishes). Rod-shaped myocytes were defined as those in which the length of the cell was at least 2 times its width with an overall linear morphology.