Modulation of (−)DOB-induced Fos-like immunoreactivity in medial prefrontal cortex and somatosensory cortex
Animals (n = 6-8 per group) were given LY341495 (3.0 mg/kg i.p.) or saline 10 min before BINA (65 mg/kg i.p.) or vehicle 40 min before (−)DOB (1.0 mg/kg s.c.) or saline. Shown is the number of Fos-LI-positive cells in the region of the mPFC or SSC analyzed. BINA attenuated the (−)DOB-induced Fos expression in the mPFC but not the SSC, an effect reversed by the mGlu2/3 receptor antagonist LY341495. BINA did not significantly alter Fos expression alone. LY341495 significantly increased saline-induced but not (−)DOB-induced Fos expression.
| Drug Treatments, mg/kg | Number of Fos-LI-Positive Cells | |
|---|---|---|
| mPFC | SSC | |
| Saline-vehicle-saline | 29.1 ± 4.2 | 0.13 ± 0.085 |
| LY341495-vehicle-saline | 50.3 ± 7.2*† | 0.57 ± 0.25 |
| Saline-BINA-saline | 30.4 ± 4.0 | 1.2 ± 0.38 |
| Saline-vehicle-DOB | 51.4 ± 4.1*† | 37.0 ± 5.4* |
| Saline-BINA-DOB | 30.4 ± 4.0 | 46.3 ± 10.5* |
| LY341495-vehicle-DOB | 65.0 ± 7.4*† | 52.4 ± 12.2* |
| LY341495-BINA-DOB | 58.4 ± 6.3*† | 67.8 ± 14.9* |