TABLE 3

Kinetic analysis of UGT1A6 control and 6H371A and 6E379A mutant forms

Constant scopoletin concentration (2 mM). UDPGA kinetics was measured using nine UDPGA concentrations between 25 and 20,000 μM and 2000 μM scopoletin (n = 3). Numbers in parentheses indicate the ratio K_m(mutant)/K_m(control) (i.e., to indicate how much the mutant K_m value increased).