MAG levels in Neuro-2a cells following DGL-α overexpression or silencing
MAG | DGLα Overexpression | DGLα Silencing | Nontransfected | ||||||
|---|---|---|---|---|---|---|---|---|---|
| pEF | α | LacZ-i | Unrl-i | α-i | |||||
| pmol/mg protein | |||||||||
| 16:0 (m/z 353) | 278 ± 8 | 275 ± 4 | 518 ± 23 | 744 ± 36 | 598 ± 55 | 480 ± 28 | |||
| 18:0 (m/z 381) | 51 ± 9 | 37 ± 2 | 18 ± 2 | 20 ± 1 | 34 ± 5 | 12 ± 2 | |||
| 18:1 Δ9 (m/z 379) | 206 ± 18 | 399 ± 20*** | 430 ± 12 | 570 ± 48 | 445 ± 23 | 461 ± 18 | |||
| 18:2 Δ9,12 (m/z 377) | 29 ± 3 | 68 ± 2*** | 34 ± 3 | 40 ± 3 | 42 ± 7 | 46 ± 6 | |||
| 18:3 Δ9,12,15 (m/z 375) | 9 ± 2 | 36 ± 23 | 22 ± 1 | 29 ± 3 | 36 ± 2 | 37 ± 4 | |||
| 20:3 Δ8,11,14 (m/z 403) | 59 ± 7 | 166 ± 5*** | 72 ± 4 | 92 ± 7 | 83 ± 12 | 174 ± 5 | |||
| 20:4 Δ5,8,11,14 (m/z 401) | 322 ± 23 | 1001 ± 35*** | 325 ± 22 | 327 ± 24 | 223 ± 19* | 484 ± 29 | |||
| 20:5 Δ5,8,11,14,17 (m/z 399) | 12 ± 1 | 34 ± 1*** | 13 ± 2 | 15 ± 1 | 10 ± 1 | 29 ± 2 | |||
| 22:6 Δ4,7,10,13,16,19 (m/z 425) | 10 ± 1 | 23 ± 1*** | 17 ± 1 | 23 ± 1 | 21 ± 1 | 15 ± 1 | |||
↵* P < 0.05; *** P < 0.001 vs. pEF, Student's t test (n = 4); or one-way analysis of variance followed by Dunnett's test (n = 4).