TABLE 3

Unesterified fatty acid levels in Neuro-2a cells after DGL-α overexpression or silencing


Fatty Acid

DGLα Overexpression

DGLα Silencing

Nontransfected
pEF
α
LacZ-i
Unrl-i
α-i
pmol/mg protein
16:0 (m/z 255) 293 ± 7 311 ± 11 149 ± 3 157 ± 5 140 ± 3 129 ± 4
18:0 (m/z 283) 414 ± 19 487 ± 9* 243 ± 5 245 ± 10 220 ± 7 262 ± 7
18:1 Δ9 (m/z 281) 158 ± 1 211 ± 6*** 127 ± 4 129 ± 3 96 ± 3** 121 ± 7
18:2 Δ9,12 (m/z 279) 10 ± 1 13 ± 0.2* 8.0 ± 0.3 6.6 ± 0.3 5.1 ± 0.2** 5.5 ± 0.3
18:3 Δ9,12,15 (m/z 277) 0.8 ± 0.1 0.7 ± 0.1 0.4 ± 0.0 0.4 ± 0.0 0.3 ± 0.0 0.3 ± 0.0
20:3 Δ8,11,14 (m/z 305) 3.5 ± 0.1 4.8 ± 0.2** 3.5 ± 0.2 3.3 ± 0.1 2.4 ± 0.2*(*) 3.0 ± 0.2
20:4 Δ5,8,11,14 (m/z 303) 11 ± 0.5 19 ± 0.7*** 10 ± 0.6 7.7 ± 0.3 5.8 ± 0.4*(*) 6.4 ± 0.5
20:5 Δ5,8,11,14,17 (m/z 301) 3.7 ± 0.4 4.8 ± 0.3 2.5 ± 0.1 2.2 ± 0.1 2.6 ± 0.1 7.2 ± 0.6
22:6 Δ4,7,10,13,16,19 (m/z 327)
0.5 ± 0.0
0.3 ± 0.0
0.2 ± 0.0
0.2 ± 0.0
0.3 ± 0.0
0.3 ± 0.0
  • * P < 0.05; ** P < 0.01; *** P < 0.001 vs. pEF, Student's t test (n = 4); or one-way analysis of variance followed by Dunnett's test (n = 4).