TABLE 2

Molecular composition of 1-acyl-2-arachidonoyl-sn-glycerol species in nontransfected Neuro-2a cells, as assessed by ion-trap LC/MSn analysis


Acyl Group in sn-1

Precursor Ion [MNa]+

Product Ion [M-R2COOH]+

[DAG]

Proportion
m/z nmol/mg protein % moles
18:0 667.8 341.5 8.8 ± 1.5 82
18:1 665.8 339.5 1.2 ± 0.5 11
16:0 639.8 313.5 0.53 ± 0.06 5
22:6 711.8 385.5 0.11 ± 0.02 1
20:4 687.8 361.5 0.085 ± 0.003 0.8
14:0 611.8 285.5 0.021 ± 0.003 0.2
18:2
Trace
Trace

Trace