TABLE 1

Primer and probe sequences for real-time quantitative RT-PCR measurements of rat WT AHR and rat splice-variant transcripts.

WT AHR
IV AHR
DV AHR
Sequence NM_013149 AF082125a/AF082126b AF082124
Forward ATGGTCAGTCCTCAGGCGTACTA ATGGTCAGTCCTCAGGCGTACTA AACTCACAGTCAGCCATGTTTCAG
Reverse AATGCTCGGACTCTGAAACTTGC TCCCTGTAGAAAGCCCTTTTCTTGC ATATCAGGAAGAGGCTGGGCTTC
Probe CCATGTCCATGTACCAGTGCCAGGCAGG CCATGTCCATGTACCAGTGCCAGGCAGG CCAGGCGAGGGAGGTGAGCAGCAGTC
Product size
141
151
130

  • ↵ a Alternatively spliced short insertion variant

  • ↵ b Alternatively spliced longer insertion variant