TABLE 1

Transcellular transport of phytoestrogens across MDCK II monolayers expressing mBcrp and hBCRP and across LLC-PK1 monolayer expressing mMdr1a Data are taken from Figs. 1 and 2. Each value represents the mean ± S.E. A to B represents the permeability of the transcellular transport in the apical to basal direction. B to A represents the permeability of the transcellular transport in the basal to apical direction.


Compound and Cell Line

Permeability

Flux Ratio
A to B
B to A
μl/h/well
Daidzein
   MDCKII/GFP 17.4 ± 0.2 23.7 ± 0.3 1.36
   MDCKII/hBCRP 12.9 ± 0.7 53.2 ± 4.1 4.12
   MDCKII/mBcrp 14.7 ± 0.3 44.1 ± 0.8 3.00
   LLC-PK1 26.7 ± 1.2 49.9 ± 1.0 1.87
   L-Mdr1a 27.9 ± 0.8 47.3 ± 1.0 1.70
Genistein
   MDCKII/GFP 13.0 ± 0.3 15.7 ± 0.4 1.21
   MDCKII/hBCRP 12.2 ± 0.8 28.8 ± 1.0 2.36
   MDCKII/mBcrp 12.5 ± 0.5 25.6 ± 0.3 2.05
   LLC-PK1 54.0 ± 2.3 77.5 ± 0.8 1.44
   L-Mdr1a 54.2 ± 1.1 76.0 ± 1.8 1.40
Coumestrol
   MDCKII/GFP 2.80 ± 0.04 4.08 ± 0.18 1.40
   MDCKII/hBCRP 3.13 ± 0.15 10.2 ± 0.6 3.26
   MDCKII/mBcrp 3.29 ± 0.23 8.75 ± 0.41 2.66
   LLC-PK1 11.3 ± 0.4 18.7 ± 0.4 1.65
   L-Mdr1a
18.9 ± 0.5
30.9 ± 0.6
1.63