Compound B-treated PAI-1 does not displace PAB from uPA active site PAB added to buffer or buffer containing compound B served as the background measurements for samples 1 to 3 and 4 to 6, respectively. Pretreatments at room temperature (5 min) are shown by grouping of reagents within parentheses. All other additions were placed at room temperature for 5 min before analysis of the Fl signal (excitation, 325 nm; emission, 355 nm). Each value shows mean (S.E.M.), n = 3.
Samples | Fl Signal (Normalized for PAB Alone) | |
|---|---|---|
| 1 | PAB + uPA | 27.90 (2.35) |
| 2 | PAB + PAI | 0.05 (0.13) |
| 3 | (PAB + uPA) + PAI | 0.21 (0.13) |
| 4 | (PAB + uPA) + compound B | 24.24 (2.47) |
| 5 | PAB + (PAI + compound B) | 2.20 (0.15) |
| 6 | (PAB + uPA) + (PAI + compound B) | 24.31 (2.08) |