DHEAS sensitivity of UNC-49 GABAA receptors
n = 4 unless otherwise indicated.
Subunit | DHEAS IC50 | Slope Coefficient |
|---|---|---|
| μM | ||
| UNC-49B | 500i | 1.1 ± 0.3 |
| UNC-49B/C | 29.2 ± 1.4 | 0.9 ± 0.1 |
| N305R | 98.8 ± 3.3 | 1.5 ± 0.1 |
| M1-Ra | 27.2 ± 5.2 | 1.1 ± 0.1 |
| QF-Ra,b | 26.0 ± 2.0 | 1.1 ± 0.02 |
| Xc | 43.3 ± 1.2 | 0.1 ± 0.04 |
| Yd | 47.8 ± 12.4 | 0.7 ± 0.03 |
| XYd | 14.6 ± 2.1 | 1.51 ± 0.2 |
| Y(T257F)d | 20.2 ± 2.0 (n = 6) | 0.8 ± 0.1 (n = 6) |
| Y(M258L)e | 30.6 ± 0.8 | 0.9 ± 0.03 |
| Y(S264A)f | 107.8 ± 8.8 | 0.9 ± 0.1 |
| Y(T257F/M258L)e | 51.0 ± 6.6 | 0.8 ± 0.1 |
| Y(T257F/S264A)e | 107.6 ± 23.1 | 0.1 ± 0.06 |
| Y(T257F/M258L/S264A)g | 61.7 ± 4.7 | 0.6 ± 0.04 |
| X(I265S)h | 25.1 ± 2.6 | 1.2 ± 0.1 |
↵ a Significantly different from N305R, p < 0.0001.
↵ b Not different from M1R, p > 0.05.
↵ c Significantly different from XY, p < 0.0001.
↵ d Significantly different from Y, p < 0.05, Mann—Whitney U test.
↵ e Not different from Y, p > 0.05, Mann-Whitney U test.
↵ f Significantly different from Y, p < 0.01.
↵ g Significantly different from XY, p < 0.0001.
↵ h Significantly different from X, p < 0.001.
↵ i Extrapolated.