TABLE 4

Effect of PKIα on the enhancement by formoterol of budesonide-induced GRE-dependent transcription

As show in Fig. 5, GRE BEAS-2B cells were infected with Ad5. CMV. PKIα (PKIα), an empty vector, Ad5. CMV (null) or left untreated (naive). Cells were treated with various concentrations of budesonide (30 pM-100 nM) in the absence and presence of a fixed concentration of formoterol (10 nM). After 6 h, cells were harvested for luciferase activity determination. pEC₅₀ values were calculated for budesonide in the presence or absence of formoterol. Overall 2×GRE reporter activation and the β₂-adrenoceptor-dependent enhancement over the glucocorticoid alone was also determined.