TABLE 2

Transfection efficiency, fluorescence intensity, and colocalization percentage in HeLa cells transfected with cDNA encoding GFP-fused chimeras with WGA-TRITC

Transfection efficiency, fluorescence intensity, and colocalization percentage of GFP-fused chimeras with WGA-TRITC. HeLa cells were transfected with the cDNA encoding GFP-fused chimeras, and after 24 h, cells were fixed and processed for fluorescence localization. The extent of colocalization between nucleoside transporters and WGA-TRITC was quantified using Metamorph image analysis as described under Materials and Methods. On the other hand, transfected cells were resuspended and analyzed by flow cytometry for transfection efficiency and construction expression rate. Data are expressed as the mean ± S.E.M. of three experiments carried out on different days on different batches of cells.