Inhibition of fMLP-induced migration of human neutrophils by cannabinoids
IC50 and Emax values (percentage of inhibition of fMLP-induced migration) were calculated from sigmoidal concentration-response curves constructed in GraphPad Prism 4. The data represent the mean with 95% confidence limits, n = 3 to 9.
Compound | IC50 (95% Confidence Limits) | Emax (95% Confidence Limits) |
|---|---|---|
| nM | % | |
| Endogenous lipid mediators | ||
| Virodhamine | 0.18 (0.17-0.19) | 86.3 (85.7-86.8) |
| NADA | 8.80 (4.7-16.2) | 64.0 (56.6-71.5) |
| AEA | 0.14 (0.12-0.17)a | 54.0 (53.0-55.2)a |
| 2-AG | >1000 | |
| ARA-S | >1000 | |
| PEA | >1000 | |
| LPI | >1000 | |
| Phytocannabinoids and analogs | ||
| (−)-CBD | 0.45 (0.39-0.53) | 59.5 (58.0-61.0) |
| (+)-CBD | 18.8 (15.4-23.0) | 70.0 (67.1-72.8) |
| Abnormal-CBD | 33.1 (30.0-36.4) | 69.9 (68.6-71.3) |
| O-1602 | 32.6 (24.1-44.2) | 66.6 (64.1-69.1) |
| Δ9-THC | >1000 |
|
↵ a The concentration-response curve to anandamide is biphasic. These values represent the first phase of inhibition.