TABLE 2

Turnover rates for midazolam metabolite formation of a panel of 10 mouse CYP2C enzymes

The final concentration of midazolam in the incubations was 25 μM, and 25 pmol of enzyme was used. After a preincubation of 5 min, the reaction was started by adding a NADPH-regenerating system, and the mixture was subsequently incubated for 15 min. All values are the means of three independent experiments ± S.D.


Enzyme

v
1′-OH MDZ
4-OH MDZ
min−1
CYP2C29 0.91 ± 0.04 0.019 ± 0.001
CYP2C37
CYP2C38
CYP2C39 0.10 ± 0.1
CYP2C44
CYP2C54
CYP2C55 0.016 ± 0.002
CYP2C65 0.021 ± 0.006 0.008 ± 0.003
CYP2C66
CYP2C70

0.28 ± 0.06
  • —, no metabolite detected (detection limit <0.005 min−1)