TABLE 1

Kinetic characteristics of hDAT methionine mutants

Data are presented as mean ± S.E.; n = 3.

hDAT form
[3H]DA uptake
[3H]CFT binding
Cocaine [3H]DA Uptake
IC50 [3H]CFT Binding
pmol/min/mg pmol/mg nM
WT 236 ± 13 2.54 ± 0.63 867 ± 103 170 ± 39
M111L/M116L 405 ± 41* 3.71 ± 0.56 608 ± 76 123 ± 11
M111L/M116L/M272L N.D. 1.08 ± 0.35 N.D. 106 ± 32
L80M/M111L/M116L 48 ± 3** 2.38 ± 0.69 145 ± 21* 104 ± 11
I67M/M111L/M116L
174 ± 13
2.53 ± 0.15
705 ± 147
129 ± 15

  • ND, non-detectable.

  • ↵* P < 0.01,

  • ↵** P < 0.001 relative to WT values (ANOVA).