TABLE 2

Effects of NFA on the voltage dependence of ERG channel activation

Channel
Control
1 mM NFA
Vact
k
Vact
k
 ΔVact
n
mV
hERG1 WT −29.3 ± 0.7 7.7 ± 0.1 −35.4 ± 0.9 7.6 ± 0.3 −6.1 ± 0.3** 17
rERG2 WT −8.7 ± 0.5 9.6 ± 0.2 −26.8 ± 0.6 8.9 ± 0.1 −18.1 ± 0.6 18
rERG3 WT −35.5 ± 0.4 7.0 ± 0.3 −46.6 ± 0.8 6.9 ± 0.4 −11.1 ± 0.5** 9
zERG WT −14.0 ± 1.2 7.8 ± 0.2 −30.1 ± 0.3 6.8 ± 0.2 −16.2 ± 1.1 12
N-S4 1.3 ± 1.1 10.3 ± 0.5 −15.9 ± 0.2 11.4 ± 0.6 −17.2 ± 0.8 7
S1-S4 −21.0 ± 0.9 7.9 ± 0.1 −38.1 ± 0.6 7.7 ± 0.4 −17.0 ± 0.6 10
L12 −27.2 ± 0.4 8.1 ± 0.2 −33.6 ± 0.6 8.3 ± 0.4 −6.4 ± 0.6** 9
S2 −32.6 ± 0.8 7.4 ± 0.3 −37.1 ± 0.8 7.0 ± 0.2 −4.5 ± 0.3** 6
L23 −25.7 ± 1.2 8.3 ± 0.3 −30.5 ± 0.9 7.9 ± 0.2 −4.8 ± 0.4** 6
L34 −25.8 ± 0.7 8.0 ± 0.1 −41.9 ± 0.9 8.1 ± 0.4 −16.1 ± 0.5* 14
hERG1 G514R −26.6 ± 0.4 7.5 ± 0.2 −37.2 ± 0.5 6.8 ± 0.2 −10.7 ± 0.4** 8
hERG1TTT −15.3 ± 1.3 8.9 ± 0.3 −28.8 ± 1.3 9.9 ± 0.4 −13.5 ± 1.0** 6
rERG3 G513R
−32.3 ± 1.0
10.5 ± 0.3
−49.6 ± 1.1
9.7 ± 0.6
−17.2 ± 0.8
8

  • ↵* P < 0.05;

  • ↵** P < 0.001 compared to NFA-induced shift in Vact observed for WT rERG2.