Heme ferrous-CO complex concentration during iNOS inactivation by BYK191023
The amount of ferrous heme-CO complex at each time point was calculated from the absorbance peak at 444 nm using Δϵ444-500 = 74 mM−1 cm−1. The concentration decrease correlates with the loss of heme during iNOS inactivation. The data is representative of three independent experiments.
Preincubation Time | P450 Complex |
|---|---|
| μM | |
| 0 min | 3.0 |
| 15 min | 2.3 |
| 30 min | 1.8 |
| 45 min | 1.4 |
| 60 min | 1.1 |
| 90 min | 0.8 |