TABLE 2

Effect of broad-spectrum PI3K inhibitors on IL-13-induced hyper-responsiveness in isolated tracheal rings

Tissues were preincubated for 30 min with drug vehicle [control, dimethyl sulfoxide, 0.05% (v/v)], wortmannin (100 nM), or LY249002 (10 μM). Media or IL-13 (100 ng/ml) was then added for a further 24 h before assessing contractility responses to KCl and CCh. Data are expressed as mean Emax ± S.E.M for n = 5 to 7 rings from different animals.

KCl CCh
mN/mg tissue
Control 1.7 ± 0.1 2.2 ± 0.1
Wortmannin 1.4 ± 0.2 1.8 ± 0.2
LY294002 1.9 ± 0.4 2.5 ± 0.4
IL-13 2.8 ± 0.3a 3.1 ± 0.2b
IL-13 + Wortmannin 1.3 ± 0.2c 1.8 ± 0.3c
IL-13 + LY294002 1.4 ± 0.1c 2.2 ± 0.3d

  • ↵ a P < 0.05 compared with control.

  • ↵ b P < 0.01 compared with control.

  • ↵ c P < 0.01 compared with tissues treated with IL-13 alone.

  • ↵ d P < 0.05 compared with tissues treated with IL-13 alone.