Validation of microarray results by real-time PCR
mRNA induction for 17 genes, found up-regulated by microarray, after a 6-h treatment with PACAP (100 nM), forskolin (25 μM), dbcAMP (1 mM), or NGF (100 ng/ml). Genes were classified in ascending order of regulation by PACAP obtained by real-time PCR.
Gene | Control | S.E.M. | PACAP | S.E.M. | Forskolin | S.E.M. | dbcAMP | S.E.M. | NGF | S.E.M. |
|---|---|---|---|---|---|---|---|---|---|---|
| Gata2 | 1.19 | 0.08 | 1.06 | 0.11 | 3.21 | 0.32 | 1.78 | 0.41 | 0.69 | 0.05 |
| Nrp1 | 1.23 | 0.25 | 1.08 | 0.13 | 0.41 | 0.00 | 0.42 | 0.03 | 5.26 | 0.62 |
| Pac1 | 1.03 | 0.11 | 1.23 | 0.13 | 0.77 | 0.05 | 0.66 | 0.06 | 7.20 | 0.81 |
| Anx2 | 1.06 | 0.05 | 1.35 | 0.16 | 1.29 | 0.04 | 1.70 | 0.19 | 1.54 | 0.37 |
| Homer2 | 1.08 | 0.06 | 2.18 | 0.13 | 2.08 | 0.12 | 1.10 | 0.37 | 1.56 | 0.21 |
| Por | 0.97 | 0.06 | 2.46 | 0.28 | 2.09 | 0.10 | 2.58 | 0.25 | 3.52 | 0.56 |
| Akr1b8 | 1.06 | 0.05 | 2.73 | 0.36 | 1.80 | 0.19 | 4.06 | 1.49 | 3.31 | 0.96 |
| Vil2 | 1.10 | 0.11 | 2.86 | 0.26 | 2.50 | 0.09 | 3.40 | 0.47 | 2.31 | 0.26 |
| Hspb8 | 1.06 | 0.05 | 2.95 | 0.16 | 2.24 | 0.10 | 1.25 | 0.42 | 1.88 | 0.19 |
| Egr1 | 1.29 | 0.30 | 4.04 | 0.36 | 2.30 | 0.12 | 4.22 | 0.66 | 48.60 | 13.83 |
| Glrx | 1.10 | 0.06 | 6.42 | 0.33 | 3.57 | 0.22 | 4.19 | 0.26 | 1.14 | 0.08 |
| Ptp4a1 | 1.21 | 0.16 | 6.64 | 0.45 | 4.53 | 0.15 | 6.61 | 1.78 | 2.85 | 0.39 |
| Gas1 | 0.87 | 0.14 | 7.45 | 1.63 | 6.51 | 0.66 | 8.62 | 3.09 | 0.57 | 0.20 |
| Azin | 1.11 | 0.08 | 11.44 | 2.33 | 8.69 | 0.44 | 3.93 | 1.36 | 1.37 | 0.14 |
| Odc | 1.01 | 0.06 | 12.76 | 1.75 | 5.69 | 0.62 | 7.98 | 1.89 | 7.19 | 1.45 |
| Ier3 | 1.11 | 0.07 | 33.88 | 3.58 | 14.34 | 1.02 | 15.80 | 1.21 | 3.17 | 0.77 |
|
Rgs2
| 1.35 | 0.10 | 76.70 | 12.83 | 25.66 | 3.10 | 29.31 | 4.61 | 9.67 | 1.24 |
Gata2, GATA binding protein 2; Nrp1, neuropilin 1; Pac-1, adenylate cyclase activating polypeptide 1 receptor 1; Anxa2, annexin A2; Homer2, homer homolog 2 (Drosophila); Por, P450 (cytochrome) oxidoreductase; Akr1b8, aldo-keto reductase family 1, member B8; Vil2, villin 2; Hspb8, 22-kDa heat shock protein 8; Egr1, early growth response 1; Glrx, glutaredoxin; Ptp4a1, protein tyrosine phosphatase 4a1; Gas1, growth arrest specific 1; Azin1, antizyme inhibitor 1; Odc1, ornithine decarboxylase, structural 1; Ier3, immediate early response 3; Rgs2, regulator of G-protein signaling 2.