Specific activities of the membrane, 2% Triton X-100 extracts, and concentrated gradient fractions
Values are the mean ± S.E.M. of four to six separate experiments for the cortex and three separate experiments for the thalamus.
α4+/+ | α4+/− | α4−/− | β2+/+ | β2+/− | β2−/− | ||
|---|---|---|---|---|---|---|---|
| fmol/mg protein | |||||||
| Cortex | |||||||
| Membrane | 64.9 ± 7.2 | 33.9 ± 3.3 | 2.5 ± 1.3 | 76.4 ± 3.9 | 41.4 ± 2.7 | 2.0 ± 1.7 | |
| 2% Triton X-100 | 66.7 ± 10.6 | 34.6 ± 1.6 | 3.1 ± 0.8 | 76.5 ± 14.2 | 37.7 ± 4.6 | 1.1 ± 0.1 | |
| Concentrated gradient fraction | 222 ± 32.8 | 116 ± 17.1 | 12.1 ± 3.9 | 247.5 ± 21.3 | 123.8 ± 12.3 | 2.4 ± 2.0 | |
| Thalamus | |||||||
| Membrane | 187.8 ± 23.8 | 105.0 ± 20.3 | 18.5 ± 9.2 | 177.4 ± 10.6, | 106.4 ± 15.2 | 10.0 ± 3.4 | |
| 2% Triton X-100 | 149.5 ± 26.5 | 89.9 ± 19.9 | 11.8 ± 4.0 | 170.3 ± 27.3 | 90.5 ± 2.3 | 9.5 ± 5.5 | |
| Concentrated gradient fraction | 227.1 ± 30.5 | 138.8 ± 13.1 | 25.75 ± 2.3 | 288.6 ± 21,94 | 185.4 ± 17.2 | 22.3 ± 1.3 | |