Increased Agonist Affinity | Recruits Arrestin-GFP | Increased Acid/Salt Resistance | Co-IPS | Arrestin-Dependent Desensitization | Increased Internalization | |||
---|---|---|---|---|---|---|---|---|
TRH receptors coexpressed with wild-type arrestin | ||||||||
Wild type | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||
371Stop | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||
4Ala | ++++ | ++++ | +++ | ++ | − | − | ||
4AlaStop | +++ | − | − | − | − | − | ||
Arrestins coexpressed with wild-type TRH receptor | ||||||||
Wild type | ++++ | ++++ | ++++ | ++++ | ++++ | |||
†LIELD/F391A | ++++ | ++ | N.D. | ++++ | − | |||
†Hinge | +++ |
| ++ | ++ | − | − |
N.D., not determined; ++++, very strong; +++, strong; ++, moderate; −, negligible.