PCR primer sequences
The restriction enzyme recognition sequence is underlined. Primer names beginning with 5 are forward primers, and those beginning with 3 are reverse primers.
Primer Name | Sequence (5′ to 3′) |
|---|---|
| 5′mENT1 | GCA AGA GCC AGA GGG AGG GAG |
| 3′mENT1-Kpn1 | CTT GTT AAG GGC ACT TGT GTG AGG TAC CCG GC |
| 5′HindIII-mENT1 | GGC CAA GCT TAT GAC AAC CAG T |
| 5′hENT1 | GGG AAC TAG TAG AAC ACC ATC ACC ATG ACA AC |
|
3′hENT1
| TCC TTC CTG TTC CGG GCA ATT GTA TCG ATA A |