Second-order rate constants (k2) for decreases in BZD potentiation and changes in I-GABA after MTSEA-biotin modification of mutant receptors
The concentrations of MTSEA-Biotin used were variable depending on the mutant and are listed under Materials and Methods. Values are mean ± S.D.
Receptor | BZD Potentation k2 (M−1 s−1) | n | I-GABA k2 (M−1 s−1) | n |
|---|---|---|---|---|
| α1D97Cβ2γ2 | 20.5 ± 5.7 | 6 | ||
| α1H101Cβ2γ2 | 2194.6 ± 354.1 | 6 | 122.3 ± 10.8* | 5 |
| α1N102Cβ2γ2 | 46.7 ± 5.5 | 7 | 52.9 ± 22.1 | 8 |
| α1S106Cβ2γ2 | 28.9 ± 8.2 | 6 | 11.3 ± 3.9* | 7 |
| α1H101Cβ2 |
|
| 121.4 ± 40.4 | 5 |
↵* The decrease in BZD potentiation was significantly faster than the increase in I-GABA (P < 0.05).